Molecular mechanism of MexR-mediated regulation of MexAB–OprM efflux pump expression inPseudomonas aeruginosa

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Effect of Mutation in Efflux Pump Regulatory Protein (MexR) of Pseudomonas aeruginosa: A Bioinformatic Study

ABSTRACT            Background and Objectives: Pseudomonas aeruginosa is an important non-fermenting gram-negative hospital-acquired pathogen. Treatment of P. aeruginosa infections has become more challenging due to overexpression of efflux pumps. The aim of the present study was to apply in silico analysis to evaluate the structure of the effl...

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Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit

Background and Aims: Pseudomonas aeruginosa is one of the most important pathogens of nosocomial infections, especially in the ICU (Intensive Care Unit), which has resistance to a wide range of antibiotics, especially Carbapenems. Among the most important resistance mechanisms of this bacteria against carbapenems are MexAB-OprM efflux pump. Therefore, the aim of this study was to evaluate the g...

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The Pseudomonas aeruginosa multidrug efflux regulator MexR uses an oxidation-sensing mechanism.

MexR is a MarR family protein that negatively regulates multidrug efflux systems in the human pathogen Pseudomonas aeruginosa. The mechanism of MexR-regulated antibiotic resistance has never been elucidated in the past. We present here that two Cys residues in MexR are redox-active. They form intermonomer disulfide bonds in MexR dimer with a redox potential of -155 mV. This MexR oxidation leads...

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Recent advances toward a molecular mechanism of efflux pump inhibition

Multidrug resistance (MDR) in Gram-negative pathogens, such as the Enterobacteriaceae and Pseudomonas aeruginosa, poses a significant threat to our ability to effectively treat infections caused by these organisms. A major component in the development of the MDR phenotype in Gram-negative bacteria is overexpression of Resistance-Nodulation-Division (RND)-type efflux pumps, which actively pump a...

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ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 2001

ISSN: 0378-1097,1574-6968

DOI: 10.1111/j.1574-6968.2001.tb10492.x